1. Go to the SSPSPredictor page: "Submit job"

2. Enter a Uniprot Entry or a sequence

If your input is a Uniprot Entry, we will retrieve the structure and sequence from AlphaFoldDB. This method is relatively fast (e.g., Q5VSL9 with a length of 837 completes within 10 seconds).

Otherwise, if the input is a sequence, we will use LocalColabFold for structure prediction. This method is relatively slower (e.g., Q13148 with a length of 414 residues takes approximately 6 minutes).

3. Press SUBMIT button, and then you can get JOB STATUS page:

This page will refresh every 5 seconds until the task is completed, then it will automatically redirect to the RESULTS page.

4. Get RESULTS page:

The first line is the label of the queried protein.

The second line is the predicted score

Next is the visualization of the structure.

At the bottom is the visualization of key amino acids involved in driving phase seperation.