AFP011000

General Information

Protein Name Ice-structuring protein
UniProt ID P09031
Nucleotide Sequence ID in NCBI X06356
Protein Sequence ID in NCBI CAA29655.1
Species Myzopsetta ferruginea (Yellowtail flounder) (Platessa ferruginea)
Sequence Length 48
Sequence
Structure AFDB ID: AF-P09031-F1-model_v4
Solvent Accessible Surface Area Total SASA 6547.92 Ų Polar SASA 2032.85 Ų Apolar SASA 8580.77 Ų

AFP011000000

Mutation Wild Type
Sequence
Thermal Hysteresis
PMID 8762146
DOI 10.1002/pro.5560050617
Protein Name Yellowtail Flounder AFP
Thermal Hysteresis Fig.5 Thermal hysteresis activity as a function of AFP concentration. Activity curves for winter flounder HPLC-6 and yellowtail flounder AFP were taken from Scott et al. (1987). Thecurve for AFP9 was based on serial dilutions of a stock 10 mg/mL solution. Each point is the average of at least three determinations.Standard deviations are shown by the vertical bars.
Thermal Hysteresis
PMID 3665937
DOI 10.1111/j.1432-1033.1987.tb13462.x
Protein Name Yellowtail Flounder AFP
Thermal Hysteresis Fig.4 Activity curves relating AFP concentrations and thermal hysteresis value. The activity curves for winter flounder (----) and yellowtail flounder (-) AFPs were determined using samples purified from plasma as described by Kao et al. [I7]
Ice Binding Sites
PMID 8762146
DOI 10.1002/pro.5560050617
Protein Name Yellowtail Flounder AFP
IBS The Ice-Binding Site (IBS) may contains T2,D4,T13,T24,T35 and T46.
Brief description
PMID: 8762146
DOI: 10.1002/pro.5560050617
1. Yellowtail flounder's major serum AFP has four 11-amino acid repeats.
2. It is less active than winter flounder's three-repeat AFP (HPLC-6) on a mg/mL basis but comparable on a molar basis, and far less active than winter flounder's four-repeat AFP (AFP9).
3. This is likely due to lacking supporting i+3 ice-binding residues in three repeats and different ice-binding motifs1-8,1-13 and 1-52.
PMID: 3665937
DOI: 10.1111/j.1432-1033.1987.tb13462.x
1. The cDNA encoding the major serum antifreeze protein in the yellowtail flounder was cloned, and its sequence shows differences from the winter flounder AFP, including an additional 11-amino-acid repeat and fewer hydrophilic ice-binding residues.
2. On a mass basis, the yellowtail flounder antifreeze is only 80% as effective as the winter flounder antifreeze at depressing the freezing temperature of aqueous solutions, probably due to the reduced number of hydrophilic ice-binding residues per molecule.
3. Variation in the number of 11-amino-acid repeats in AFPs might arise by unequal crossing over during recombination within the AFP multigene family.