1. lssAFP is highly expressed in peripheral tissues such as the skin, dorsal fin, and gill filaments, and may play a role in protecting against low temperatures in these tissues.
2. r-lssAFP(38) has antifreeze activity, and its activity is slightly higher than that of wfsAFP-2. The ice crystal morphologies of both are typical bipyramidal forms.
3. The longhorn sculpin (Myoxocephalus octodecemspinosus) produces skin-type, type I AFPs and type IV serum AFPs. This is the first discovery that a fish species can produce two different biochemical classes of antifreeze proteins.
AFP011003
General Information
| Protein Name | Longhorn sculpin skin-type antifreeze protein |
| UniProt ID | Q9DF18 |
| Nucleotide Sequence ID in NCBI | AF306348 |
| Protein Sequence ID in NCBI | AAG22048.1 |
| Species | Myoxocephalus octodecemspinosus (Longhorn sculpin) (Cottus octodecemspinosus) |
| Sequence Length | 38 |
| Sequence | |
| Structure | AF2 predicted Structure: AFP011003 |
| Solvent Accessible Surface Area | Total SASA | 2688.72 Ų | Polar SASA | 1086.43 Ų | Apolar SASA | 3775.15 Ų |
AFP011003000
| Mutation | Wild Type |
| Sequence |
Ice crystal morphology
| PMID | 11136728 |
| DOI | 10.1074/jbc.M009293200 |
| Protein Name | r-lssAFP(38) |
| Ice crystal morphology | Fig.4.A Production of active recombinant AFP from the lssAFP clone. A, final purification step for recombinant longhorn sculpin skintype AFP, r-lssAFP(38). C4 reverse-phase HPLC profile, 20-45% acetonitrile in 0.1% trifluoroacetic acid (60 min), the r-lssAFP(38) peak is indicated by an asterisk (*). Inset displays the ice crystal morphology in the presence of 4.65 mM r-lssAFP(38) in 0.1 M ammonium bicarbonate. |
Thermal Hysteresis
| PMID | 11136728 |
| DOI | 10.1074/jbc.M009293200 |
| Protein Name | r-lssAFP(38) |
| Thermal Hysteresis | Fig.4.B activity curve of r-lssAFP(38) compared with wfsAFP-2 (13). |
Brief description
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PMID: 11136728
DOI: 10.1074/jbc.M009293200 |