AFP011006

General Information

Protein Name Skin-type antifreeze polypeptide AFP-2
UniProt ID Q9DF23
Nucleotide Sequence ID in NCBI AF305502
Protein Sequence ID in NCBI AAG25982.1
Species Myoxocephalus scorpius (Shorthorn sculpin) (Cottus scorpius)
Sequence Length 92
Sequence
Structure AF2 predicted Structure: AFP011006
Solvent Accessible Surface Area Total SASA 5003.94 Ų Polar SASA 1736.02 Ų Apolar SASA 6739.96 Ų

AFP011006000

Mutation Wild Type
Sequence
Ice crystal morphology
PMID 9722537
DOI 10.1074/jbc.273.36.23098
Protein Name shorthorn sculpin AFP sssAFP2
Tag Hexahistidine
Ice crystal morphology Fig.5 His-sssAFP-2 is an active antifreeze peptide. A and B, shown is ice crystal morphology in the presence of His-sssAFP-2. A, 0.09 mM His-sssAFP-2; B, 1.12 mM His-sssAFP-2.
Thermal Hysteresis
PMID 9722537
DOI 10.1074/jbc.273.36.23098
Protein Name shorthorn sculpin AFP sssAFP2
Tag Hexahistidine
Thermal Hysteresis Fig.5 His-sssAFP-2 is an active antifreeze peptide. C and D, thermal hysteretic activity of His-sssAFP-2 (▲) and wflAFP-6 (■), measured in molar concentration (C) and in mg/ml (D).
Ice Binding Sites
PMID 9722537
DOI 10.1074/jbc.273.36.23098
Protein Name shorthorn sculpin AFP sssAFP2
Tag Hexahistidine
IBS It is proposed that polar residues in the 11-residue repeats (such as Pr in the Pr-X2-Pr-X7 repeats) may interact with the ice-crystal lattice through hydrogen bonding.
Brief description
PMID: 9722537
DOI: 10.1074/jbc.273.36.23098
1. It was shown that sssAFP-2 mRNA is expressed in various tissues such as skin, dorsal fin, brain, and gill filament, but not in the liver, and exhibits significant seasonal variation.
2. The recombinant protein His-sssAFP-2 was constructed and purified. It has an α-helical structure with a helix content of approximately 74% at 0 ℃. Its thermal hysteresis activity is concentration-dependent. Compared with the winter flounder liver-type antifreeze protein wflAFP-6, it has similar activity on a molar basis but lower activity on a mg/ml basis.