1. Two major pro-antifreezes corresponding to the precursors for AFP-6 and AFP-8 were isolated and characterized from winter flounder liver.
2. The pro-antifreezes were synthesized in the liver seasonally, starting in October and ceasing in March.
3. Both proAFP-6 and proAFP-8 showed antifreeze activity, but their activities were lower than those of the mature AFP.
AFP011008
General Information
| Protein Name | Antifreeze protein |
| Gene Name in Uniprot | AFP |
| UniProt ID | Q9YH51 |
| Species | Pseudopleuronectes americanus (Winter flounder) (Pleuronectes americanus) |
| Sequence Length | 59 |
| Sequence | |
| Structure | AFDB ID: AF-Q9YH51-F1-model_v4 |
| Solvent Accessible Surface Area | Total SASA | 5713.38 Ų | Polar SASA | 2048.01 Ų | Apolar SASA | 7761.39 Ų |
AFP011008000
| Mutation | Wild Type |
| Sequence |
Thermal Hysteresis
| PMID | 3769927 |
| DOI | 10.1111/j.1432-1033.1986.tb09966.x |
| Protein Name | winter flounder proAFP-8 |
| Thermal Hysteresis | Fig.3 The antifreeze activity of flounder pro-antifreezes plotted against concentration by mass ( A ) and against molar concentration ( B ) . Various amounts of proAFP-6, proAFP-8 and AFP-6 were dissolved in 0.01 M NH4HC03. Thermal hystereses of these materials were measured using a nanoliter osmometer. Each point, which is an average of four measurements, has a deviation of ±0.01℃. |
Brief description
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PMID: 3769927
DOI: 10.1111/j.1432-1033.1986.tb09966.x |